While gene expression profiling is a widely used and highly proven technology for the dissection of disease and the unraveling of underlying pathological processes, proteome profiling is, in contrast, still in its infancy.
CRT's monoclonal antibody proteomics tools are specifically designed to fill the gap!
CRT's goal is to provide the research community with comprehensive mAB libraries generated against the epitome of the human proteome. At CRT we use our robust technology to produce increasingly large sets of mAB libraries that will cover the entire human plasma epitome.
Our mAB libraries provide the tools that enable the research community to discover new disease specific biomarkers via robust antibody micro-array profiling of human tissue samples.
The mAB libraries are ready for profiling your samples via micro-array technology provided by our partners.
How to use the CRT antibody arrays
Step 1. Sample preparation and proteome profiling.
Plasma samples from clinical cohorts require sample preparation. During the sample preparation process plasma proteins are labelled and the labelled samples are incubated with the microarray slides under controlled conditions. For optimal signals the plasma proteome may be subjected to depletion to remove abundant proteins (see our sample preparation services).
Step 2. Characterization of cognate targets of individual mABs.
Comparison of plasma proteome profiles will result in the identification of mABs that detect quantifiable differences between clinically and/or biologically different sample sets. As needed, BSI and our partners will provide the user with available data on each mAB (e.g., protein ID, epitope/mimitope, V region sequence of light and heavy chains) and a sufficient quantity of purified mABs to further characterize the cognate antigen recognized by the mABs by the user (see our mAB production and characterization services).
Our array printing team is highly skilled in working with microarray printing technology and
system integration. We work closely with our clients to design array formats that fit your
needs. Our services also include printing on 3-D glass substrates.
Printing conditions are optimised for each new sample printed to ensure adequate sample immobilisation and to preserve the activities of each unique sample. Our rigorous quality control procedures ensure printed arrays of superior spot morphology, consistency and uniformity.